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Segmented double-stranded genomic RNA viruses in fecal samples from broiler chicken BJM
Tamehiro,Claudia Yurika; Alfieri,Alice Fernandes; Médici,Cristina; Alfieri,Amauri Alcindo.
Segmented double-stranded RNA (dsRNA) viruses were identified by polyacrylamide gel electrophoresis (PAGE) technique in fecal samples from broiler chicken. A total of 378 fecal samples from 1-7 weeks old chickens were analyzed. dsRNA with migration profile characteristic of avian rotavirus (AvRV), reovirus (ARV) or picobirnavirus (PBV) was identified in 32 (8.5%), 7 (1.8%) and 13 (3.4%) samples, respectively. AvRV and ARV occurred more frequently in chickens up to 1 month old and were related with enteritis signs. Considering only fecal samples of chickens with diarrhea, the AvRV was detected in 37.8% (14/37) and the ARV in 13.5% (5/37) of analyzed samples. AvRV was identified in only 1.5% (4/274) and ARV was not detected in normal feces collected from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian rotavirus; Avian reovirus; Avian picobirnavirus; Broiler chickens; Diarrhea; PAGE.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400013
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The occurrence of Orthoreovirus, Rotavirus and chicken anemia virus in chickens of the poultry industry in Minas Gerais, Brazil Arq. Bras. Med. Vet. Zootec.
Rios,R.L.; Marin,S.Y.; Gomes,A.M.; Resende,J.S.; Bonfim,M.R.Q.; Gomes,A.D.; Resende,F.M.S.; Horta,R.S.; Resende,M.; Martins,N.R.S..
Fifty-four fecal samples taken from broiler chickens from 1 to 45 days of age, and of pullets from 10 to 13 weeks of age, original from eight different poultry regions in the state of Minas Gerais, Brazil, were collected from March 2008 to January 2010 for avian Orthoreovirus (ARV) and avian Rotavirus (AvRV) analyses. For the assay of ARV, RNA was immediately extracted (Trizolâ) and transcribed into cDNA for assaying in a nested-PCR with ARV-specific primers. For AvRV, polyacrylamide gel electrophoresis (PAGE) was performed with RNA extracts obtained by phenol-chloroform extraction. CAV was additionally investigated through a nested-PCR of thymus and spleen. Results found 5.55% positive for ARV and 9.25% for AvRV. Also, CAV and ARV genomes were detected in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Layer; Chicken anemia virus; CAV; Avian reovirus; ARV; Avian rotavirus; AvRV; Nested-PCR; PAGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000600030
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